Composite

Part:BBa_K2268004:Design

Designed by: Devin Camenares   Group: iGEM17_Kingsborough_NY   (2017-10-27)


p(cI)-LacZ alpha, [p(Lac)-cI] reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA was synthesized as two separate fragments and assembled together. cI repressor expression is oriented in reverse to prevent read-through transcription by upstream module (also, acts to further repress upstream expression)


Source

DNA was synthesized by IDT; sequences obtained from component parts assembled via Gibson method.

References